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Details for China GlutathioneS-Transferase,GST

GlutathioneS-Transferase,GST 50812-37-8

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 GlutathioneS-Transferase,GST
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Specification : White freeze-dried powder or liquid
Product description : Glutathione thiol transferase (GST, 2.5.1.18) is generally formed by the aggregation of two subunits of 25-27kDa in a homologous or heterologous manner (with an isoelectric point of pH4-5). The N-terminal of its subunit has a conserved serine/tyrosine (Ser/Tyr) residue, and its hydroxyl group forms a hydrogen bond with the thiol group of glutathione (GSH), which is a specific binding site for GSH. Glutathione thiol transferase (GST, 2.5.1.18) is generally formed by the aggregation of two subunits of 25-27kDa in a homologous or heterologous manner (with an isoelectric point of pH4-5). The N-terminal of its subunit has a conserved serine/tyrosine (Ser/Tyr) residue, and its hydroxyl group forms a hydrogen bond with the thiol group of glutathione (GSH), which is a specific binding site for GSH. GST is a group of multifunctional isoenzymes widely distributed in various organisms. It is a group of enzymes related to the detoxification function of the liver, mainly present in the liver, and in trace amounts in tissues such as the kidneys, small intestine, testes, ovaries, etc. Its main function is to catalyze the coupling of electrophilic groups of certain endogenous or foreign harmful substances with the thiol group of reduced glutathione, increasing its hydrophobicity and making it easy to cross cell membranes, And after being decomposed, it is discharged from the body to achieve the purpose of detoxification. Due to the abundance of GST in the cytoplasm of liver cells, enzymes are rapidly released into the bloodstream when liver cells are damaged, leading to an increase in serum GST activity. GST is commonly used in genetic engineering to construct efficient expression vectors, serving as a molecular chaperone to co express with some difficult to express proteins, thus achieving a soluble expression; In addition, GST antibodies are often used for Western Blot and other experiments to detect whether gene co expression is achieved, so studying them is of great significance.
Uses : enzymic preparations
Synonyms : Transferase, glutathione S-;Glutathione S-transferase;Glutathione Stransferase;Glutathione transferase;Glutathione-S-transferase;Interferon gamma, human